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GPR34 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q9UPC5 |
| Other Accession | Q9R1K6, NP_001091048.1 |
| Reactivity | Human |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 43860 Da |
| Antigen Region | 232-261 aa |
| Gene ID | 2857 |
|---|---|
| Other Names | Probable G-protein coupled receptor 34, GPR34 |
| Target/Specificity | This GPR34 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 232-261 amino acids from the Central region of human GPR34. |
| Dilution | IHC-P~~1:10~50 WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | GPR34 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GPR34 |
|---|---|
| Function | G-protein-coupled receptor of lysophosphatidylserine (LysoPS) that plays different roles in immune response (PubMed:16460680). Acts a damage-sensing receptor that triggers tissue repair upon recognition of dying neutrophils (By similarity). Mechanistically, apoptotic neutrophils release lysophosphatydilserine that are recognized by type 3 innate lymphoid cells (ILC3s) via GPR34, which activates downstream PI3K-AKT and RAS-ERK signaling pathways leading to STAT3 activation and IL-22 production (By similarity). Plays an important role in microglial function, controlling morphology and phagocytosis (By similarity). |
| Cellular Location | Cell membrane; Multi-pass membrane protein |
| Tissue Location | Broadly expressed. Highly expressed on mast cells (PubMed:16460680). |
Provided below are standard protocols that you may find useful for product applications.
Background
G protein-coupled receptors (GPCRs), such as GPR34, are integral membrane proteins containing 7 putative transmembrane domains (TMs). These proteins mediate signals to the interior of the cell via activation of heterotrimeric G proteins that in turn activate various effector proteins, ultimately resulting in a physiologic response.
References
Engemaier, E., et al. Genomics 87(2):254-264(2006)
Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005)
Jacobi, F.K., et al. Hum. Genet. 107(1):89-91(2000)
Schoneberg, T., et al. Biochim. Biophys. Acta 1446 (1-2), 57-70 (1999) :
Marchese, A., et al. Genomics 56(1):12-21(1999)
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